Figure 4.

MPK6-Silenced Lines Display Enhanced Susceptibility to P. s. tomato.

(A) Four-week-old plants were inoculated with P. s. tomato, and four leaf disks per sample were harvested at 0 dpi and 3 dpi. The number of colony forming units (cfu) per sample was calculated and expressed as cfu/mL. Col-0 and MPK3ihp L5 were used as controls, along with the MPK6ihp L1, L4, and L7 lines. Experiments were done twice with similar results.

(B) Four-week-old plants were inoculated with P. s. tomato expressing avrRpm1, and four leaf disks per sample were harvested at 0 dpi and 3 dpi.

(C) Four-week-old plants were inoculated with P. s. tomato expressing avrRpt2, and four leaf disks per sample were harvested at 0, 1, and 2 dpi.

(D) Four-week-old plants were inoculated with P. s. tomato expressing avrRps4, and four leaf disks per sample were harvested at 0, 2, and 3 dpi.