Extended data Figure 2. HSC competitiveness is increased in Vav1-Cre; NrasG12D/+ mice.
a) Frequencies of CD150+CD48−LSK HSCs, CD150−CD48−LSK MPPs, and LSK cells in the bone marrow (BM, top row) and spleen (sp, bottom row) of Vav1-cre; NrasG12D/+ (G12D/+) or littermate control (con) mice (n=4) at 6-10 weeks of age. b) 5×105 donor bone marrow cells from Vav1-cre; NrasG12D/+ (G12D/+) or littermate control (con) mice at 6-10 weeks of age were transplanted into irradiated recipient mice along with 5×105 recipient bone marrow cells (3 donors/genotype were each transplanted into 4 recipients/donor). c) Secondary transplantation of 3×106 bone marrow cells from primary recipient mice in Extended data Figure 2b at 20 weeks after transplantation (2 primary recipients/genotype were each transplanted into 4 secondary recipients/primary recipient). Data represent mean±s.d.. Two-tailed student's t-tests were used to assess statistical significance. *P<0.05, **P<0.01, ***P<0.001.