Extended data Figure 5. Nras^G12D-induced changes in HSC function were not associated with the development of leukemia.

White blood counts (WBC), hemoglobulin (Hb) levels, platelet counts, and spleen masses for recipient mice from primary transplants (a; from Figure 1d), secondary transplants (b; from Figure 2a), tertiary transplants (c; from Figure 2b) and quaternary transplants (d; from Figure 2c). In all cases, these blood cell counts were collected from mice after the analysis of blood cell reconstitution was complete (at least 20 weeks after transplantation). The transplanted mice were observed for a median time of 260 (162–315) days for primary recipient mice, 194 (122–264) days for secondary recipient mice, 224 (176–336) days for tertiary recipient mice, and 280 (279–280) days for quaternary recipient mice. We never observed evidence of leukemia or MPN by histology in these mice. Across all of the experiments, only two recipients of Nras^G12D/+ cells and two recipients of control cells died spontaneously. Data represent mean±s.d.. Two-tailed student's t-tests were used to assess statistical significance and none of the comparisons showed significant difference.