Dendrogram depicting the phylogenetic relationship between the aquaporins of Touriga nacional variety cloned in this study (framed in rectangular shape), previous study (oval shape frame) [17] with the aquaporins of Pinot noir variety. Dendogram was generated by neighbor-joining method (applied to 1000 bootstrap data sets) using the MEGA5.1 program [26]. Accession numbers of presented protein sequences are: VvPnPIP1;1 (CAO41326, GSVIVT00029248001), VvPnPIP1;3 (CAO62835, GSVIVT00000433001), VvPnPIP1;4 (CAO39626, GSVIVT00026881001), VvPnPIP1;5 (CAO39627, GSVIVT00026882001), VvPnPIP2;1 (CAN75442), VvPnPIP2;2 (CAO47394, GSVIVT00036133001), VvPnPIP2;3 (CAO18152, GSVIVT00023192001), VvPnPIP2;4 (CAO21844, GSVIVT00024536001), VvPnTIP1;1 (CAO69259, GSVIVT00018548001), VvPnTIP1;2 (CAO63006, GSVIVT00000605001), VvPnTIP1;3 (CAO16745, GSVIVT00022146001), VvPnTIP1;4 (CAO21720, GSVIVT00024394001), VvPnTIP2;1 (CAO45860, GSVIVT00034350001), VvPnTIP2;2 (CAO23095, GSVIVT00012703001), VvPnTIP3;1 (CAO62035, GSVIVT00013854001), VvPnTIP4;1 (CAO44039, GSVIVT00032441001), VvPnTIP5;1 (CAO42713, GSVIVT00029946001), VvPnTIP5;2 (CAO70596, GSVIVT00019170001), VvPnNIP1;1 (CAO48005, GSVIVT00035815001), VvPnNIP2;1 (CAO15462, GSVIVT00011149001), VvPnNIP3;1 (CAO17108, GSVIVT00022377001), VvPnNIP4;1 (CAO70192, GSVIVT00007127001), VvPnNIP4;2 (CAO43338, GSVIVT00003903001), VvPnNIP5;1 (CAO62847, GSVIVT00000446001), VvPnNIP6;1 (CAO45476, GSVIVT00033750001), VvPnNIP7;1 (CAO71103, GSVIVT00019910001), VvPnSIP1;1 (CAO23510, GSVIVT00025504001) and VvPnSIP2;1 (CAO18284, GSVIVT00023346001) (for Pinot noir cultivar sequences) and VvTnPIP1;1 (HQ913643), VvTnPIP1;4 (KJ697714), VvTnPIP2;1 (KJ697715), VvTnPIP2;2 (HQ913642), VvTnPIP2;3 (KJ697716), VvTnTIP1;1 (KJ697717), VvTnTIP2;1 (HQ913640), VvTnTIP2;2 (KJ697718) and VvTnTIP4;1 (KJ697719) (for Touriga nacional cultivar sequences).