Table 3. Entry and intracellular growth of Map isolates and the reference strain K10 in ovine MDMs.
| Isolate | Host-IS1311 PCR/REA type | Entry (%) a | log10 CFU (± SD) b | n-Fold d | P value | |
| Day 0 c | Day 7 | |||||
| K10 | Cattle-C | 65.32 | 4.88 (±2.59) | 7.99 (±0.35) | 1.63 | 0.0058 e |
| 681 | Wild board- C | 73.13 | 5.33 (±0.99) | 8.01 (±0.20) | 1.50 | 0.1901 |
| 6 | Cattle-C | 67.36 | 5.86 (±2.73) | 7.89 (±1.50) | 1.34 | 0.4752 |
| 334 | Sheep-B | 75.01 | 6.05(±3.28) | 7.64 (±3.47) | 1.26 | 0.9942 |
| 622/07 | Fallow deer-C | 79.56 | 7.48 (±1.66) | 8.82 (±0.19) | 1.17 | 0.9998 |
| 711P | Goat-C | 74.91 | 5.59 (±0.80) | 6.17 (±1.10) | 1.10 | 1.0000 |
| P381 | Sheep-C | 86.39 | 7.81 (±1.36) | 8.44(±0.22) | 1.08 | 1.0000 |
| 311 | Goat-S | 77.00 | 7.75 (±2.44) | 8.19 (±2.44) | 1.05 | 1.0000 |
| 2349/06-1 | Sheep-S | 51.93 | 4.93 (±0.05) | 4.90 (±0.24) | 0.99 | 1.0000 |
| 855 | Deer-C | 74.45 | 5.86(±0.73) | 5.77 (±0.86) | 0.98 | 1.0000 |
a
Uptake was calculated as the percentage of the inoculated bacteria that was recovered from each cell lysate at day 0.
b
Values shown are means of three repeated experiments ± standard deviations (SD).
c
Day 0 = 2 h post infection.
d
Growth changes (n-fold) were calculated by dividing the number of log10 CFU at day 7 by that at day 0 for each Map isolate.
e
Indicates a significant change between day 0 and day 7 (P<0.05).