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. 2014 Aug 11;9(8):e103723. doi: 10.1371/journal.pone.0103723

Figure 2. RT-PCR and IB assays showing the changes on Hsp90α expression after burn injury at the wound edge in mice.

Figure 2

(A) mRNA level of Hsp90α was measured at each time point after burn injury by real-time PCR and normalized against GAPDH. Results showed that the mRNA level in burned skin peaked at 3∼6 h and was 20 times higher than that in control mice (p<0.05). (B) The change of Hsp90α protein level after burn injury was analyzed by western blotting (p<0.05). (C) Histogram quantified the relative Hsp90α level in (B).