Figure 2.

Medium Change–Induced Touch Response in M. truncatula.

(A) Young growing M. truncatula dmi2-1 root hair just before medium change.

(B) M. truncatula dmi2-1 root hair in the same region on the root as the one in (A), just after medium change. In just 1 min, the cytoplasm has reorganized such that it evenly surrounds the central vacuole, creating a cytoarchitecture of a full-grown root hair.

(C) Percentage of M. truncatula root hairs with a cytoarchitecture of a growing root hair before and after medium change. Before medium change, this percentage in the wild type and dmi2-1 is ∼100%. After medium change, this percentage decreases in both the wild type and dmi2-1. In time, the wild type shows recovery, whereas in dmi2-1, the typical cytoarchitecture does not recover. Monitored were six roots per wild type and dmi2-1, respectively, >50 root hairs per root. Bars in (A) and (B) = 10 μm.