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. 2014 Aug 11;9(8):e104418. doi: 10.1371/journal.pone.0104418

Figure 4. In situ detection of APOH/CDC42 and APP/NGFR interactions in the rat testis by Duolink PLA.

Figure 4

(A, E) Abundant PLA signals (red dots) were detected in the seminiferous tubules of rat testis when specific primary antibodies were used, reflecting the close proximity of APOH/CDC42 or APP/NGFR close proximity. (B, C, F, G) Negative controls, with only one primary antibody for the targeted protein-protein interaction. (D, H) Quantification of PLA signals in testis sections for APOH/CDC42 (** Student's t-test p = 0.0016) or for APP/NGFR (Student's t-test p = 0.16). Scale bars = 50 µm. A nonspecific background nuclear signal was observed for a few tubule sections (see B).