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. 2014 Aug 11;9(8):e104313. doi: 10.1371/journal.pone.0104313

Figure 2. Schematic diagram of the B. pseudomallei K96243 bprD gene and agarose gel of RT-PCR products.

Figure 2

Schematic diagram of the genomic organization of the B. pseudomallei K96243 region containing bprD (A), and RT-PCR analysis of expression of genes in this region (B). The arrows show the position and direction of genes. The positions of RT-PCR primers are indicated by black arrows. Lane 1, RT-PCR product from B. pseudomallei K96243 wild-type cDNA; lane 2, negative control for wild-type or DNase-treated wild-type RNA (to evaluate contamination of wild-type RNA with gDNA); lane 3, B. pseudomallei K96243 bprD mutant cDNA; lane 4, negative control for mutant or DNase-treated bprD mutant RNA (to evaluate contamination of mutant RNA with gDNA); lane 5, B. pseudomallei K96243 wild-type genomic DNA control; and lane 5, No DNA control.