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. 2014 Aug 11;9(8):e104313. doi: 10.1371/journal.pone.0104313

Figure 5. Photomicrographs of hematoxylin-and-eosin-stained spleens from BALB/c mice infected with B. pseudomallei.

Figure 5

The spleens were collected on day 3 from non-infected BALB/c mice (A; ×100) and mice infected with B. pseudomallei (B–F). The spleen of mice infected with the B. pseudomallei K96243 wild-type strain showed multifocal areas of inflammatory cell infiltration and neutrophil abscess formation (arrows in B; ×100). The neutrophils (arrow) and necrotic cells (arrow head) are shown at high magnification (C; ×400). Numerous multinucleated giant cells are observed (arrows in D; ×400). Mice infected with the B. pseudomallei bprD mutant showed multifocal to coalescent pyogranulomatous splenitis (E; ×100). Necrotic areas with neutrophils (arrow) are shown at high magnification (F; ×400).