Table 2. Bacterial strains used in this study.
Bacterial strain | Description | Source or Reference | ||
B. pseudomallei | 1909a | Clinical isolate from sputum of 34-year-old male with diabetes mellitus who is a rice farmer in Ubonratchathani province, Thailand | Kindly provided by Dr. Narisara Chantratita, Mahidol Oxford Tropical Medicine Research Unit (MORU), Faculty of Tropical Medicine, Mahidol University. | |
B. pseudomallei | K96243 | Clinical isolate from a patient admitted to Khon Kaen provincial hospital, Khon Kaen, Thailand | [21] | |
B. pseudomallei | K96243 | bprD mutant | K96243 derivative; DbprD; Tcr | This study |
B. pseudomallei | K96243 | bprD complemented | bprD mutant carrying pBBR1MCSbprD; Tcr Cmr | This study |
B. pseudomallei | K96243 | bprD clean mutant | K96243 derivative; DbprD | This study |
E. coli | DH5α | General cloning | [46] | |
E. coli | SM10lpir | Mobilizing strain, SM10 with a l prophage carrying the gene encoding the π protein; Cms Tcs Gms Kmr Tps Pxs | [47] | |
E. coli | S17-llpir | Mobilizing strain, S17-1 with a l prophage carrying the gene encoding the π protein; Cms Tcs Gms Kms Tpr Pxs Smr | [49] |
Cm, chloramphenicol; Gm, gentamicin; Ap, ampicillin; Tc, tetracycline; Km, kanamycin; Tp, Trimethoprim; Px, polymyxin; Sm, streptomycin; r = resistance; s = sensitive.