. 2014 Aug 11;9(8):e104313. doi: 10.1371/journal.pone.0104313

Table 3. Plasmids used in this study.

Plasmids	Description	Source or Reference
pDM4	Suicide vector; sacBR oriT oriR6K Cm^r	[48]
pDM4Δ *bprD*	pDM4::ΔbprD B. pseudomallei K96243; Cm^r	This study
pDM4Δ *bprD* :: *Tc^r*	pDM4::ΔbprD::Tc^r B. pseudomallei K96243; Cm^r Tc^r	This study
pBBR1MCS	A Cm^r bhr plasmid of 4.7 kb, and contains 16 unique cloning sites within the lacZ∼ gene, Km^r, Ap^r, Tc^r, and Gm^r	[38]
pBBR1MCS *bprD*	pBBR1MCS harboring the bprD gene	This Study
pUTminiTn5Tc	R6K-based suicide delivery plasmid; Tc^r	Kindly provided by Prof. Ben Adler

Cm, chloramphenicol; Gm, gentamicin; Ap, ampicillin; Tc, tetracycline; Km, kanamycin; Tp, Trimethoprim; Px, polymyxin; Sm, streptomycin; r = resistance; s = sensitive.