Table 4. Primers used in this study.
| GenBank accession number | Gene symbol | Gene Description | Primer name | Primer sequence | Purpose | Amplicon size (bp) |
| YP_111519.1 | BPSS1512 | Hypothetical protein | BPSS1512-F | GGACAAACGCTGGAAGTGAT | Internal region | 129 |
| YP_111519.1 | BPSS1512 | Hypothetical protein | BPSS1512-R | GTTCAGAAAGAACGCCTTGG | Internal region | 129 |
| YP_111526.1 | BPSS1520 | AraC family transcriptional regulator | BPSS1520-F | GGACGAGCTCGATTACATGC | Internal region | 153 |
| YP_111526.1 | BPSS1520 | AraC family transcriptional regulator | BPSS1520-R | GGCAGATGAAGATGCTGCTC | Internal region | 153 |
| YP_111527.1 | BPSS1521 | Hypothetical protein | BPSS1521-F | TCGATCTTCTCGCTGACCTC | Internal region | 100 |
| YP_111527.1 | BPSS1521 | Hypothetical protein | BPSS1521-R | TCAAGGAGATCCGCTTCAAC | Internal region | 100 |
| YP_111527.1 | BPSS1521 | Hypothetical protein | bprD-PFUp | TTAATTTCTAGAGCATCGGAGCAACAAGAATC | Upstream BPSS1521 | 927 |
| YP_111527.1 | BPSS1521 | Hypothetical protein | bprD-PRUp | TTATGAAGATCTTTTCCTGTCGTGAACATTGG | Upstream BPSS1521 | 927 |
| YP_111527.1 | BPSS1521 | Hypothetical protein | bprD-PFDown | TTGTCAAGATCTGCGTAACGTGTGACGTGTTG | Downstream BPSS1521 | 929 |
| YP_111527.1 | BPSS1521 | Hypothetical protein | bprD-PRDown | ATAATACCCGGGGATGAAGATGCTGCTCGATG | Downstream BPSS1521 | 929 |
| YP_111527.1 | BPSS1521 | Hypothetical protein | bprD-PFComp | ATACTTGGTACCAGGAAAGACATCATGAAGCTC | Full-length BPSS1521 | 456 |
| YP_111527.1 | BPSS1521 | Hypothetical protein | bprD-PRComp | TTAACATCTAGATCACGGCGCCGGGCGCTG | Full-length BPSS1521 | 456 |
| AY_305818.1 | 16S ribosomal RNA | 16s rRNA-F | GGCTAATACCCGGAGTGGA | Internal region | 194 | |
| AY_305818.1 | 16S ribosomal RNA | 16s rRNA-R | CTAGCCTGCCAGTCACCAA | Internal region | 194 | |
| YP_111503.1 | BPSS1496 | Hypothetical protein | BPSS1496-F | AGCGGGTCAACATCGTCTAT | Internal region | 163 |
| YP_111503.1 | BPSS1496 | Hypothetical protein | BPSS1496-R | ACGTCGTTGAAGTCGTCCTT | Internal region | 163 |
| YP_111504.1 | BPSS1497 | Hypothetical protein | BPSS1497-F | GCTCGCTGAAGTTTCTCGTC | Internal region | 151 |
| YP_111504.1 | BPSS1497 | Hypothetical protein | BPSS1497-R | TCGGCCGTATAGACCTTCTG | Internal region | 151 |
| YP_111505.1 | BPSS1498 | Hypothetical protein | BPSS1497-F | GTCATGACGGGAAAATCCAC | Internal region | 134 |
| YP_111505.1 | BPSS1498 | Hypothetical protein | BPSS1497-R | CGACGATCTGTCCATTTCCT | Internal region | 134 |
| pDM4 | BAP3771 | TAACGGCAAAAGCACCGCCGGACATCA | pDM4 MCS flanking region | 177 | ||
| pDM4 | BAP3772 | ACATGTGGAATTGTGAGCGGATAACAA | pDM4 MCS flanking region | 177 | ||
| Tcr cassette | BAP5118 | GCGTAGTCGATAGTGGCTCC | Internal region | |||
| Tcr cassette | BAP5116 | ATCAGGGACAGCTTCAAGGA | Internal region |
MCS, multiple cloning site.