Table 2. PCR primers used for Lefty sequence analysis.

Gene	Exon	Forward primer	Reverse primer	Size	Tm
LEFTY1	1	TGCCTGAGACCCTCCTGC	CCCTCACTCAGCCTCCCA	436	59.9
	2	TTTGCCCCAGAAATAGAACAGG	GACCCAGCGCCGCTTGAG	499	62.1
	3	CAACCGCACCTCCCTCAT	CATTCATTCCCACAGCACTC	513	59.2
	4	TAAATCTCCATCCCAGACGC	ACCCTCGAACACTTCAGAAACA	499	57.9
LEFTY2	1	CTCCCTCTTCCCTTCACCC	ACAGCCTCCCACAGAGTCCC	511	60.5
	2	GCCTGGCTGCCAGCTCAG	GACCCAGCGCCGCTTGAG	462	62.7
	3	CAACCGCACCTCCCTCATC	GCAATCGCTGGCATCCTG	570	61.7
	4	CCTCCCAGGTGCCCACTA	GGGATGGAGTAACTTGCTAA	549	56.5