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. 2004 Apr;16(4):977–992. doi: 10.1105/tpc.020156

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Copyright © 2004, American Society of Plant Biologists

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Figure 7. — Purification of Native GPPS from A. majus Upper and Lower Petal Lobes.

(A) Anion exchange chromatography (Mono Q) of GPPS activity containing fractions after initial DEAE-Sepharose chromatography. Absorbance at 280 nm, prenyltransferase activities (in cpm), and the NaCl gradient are indicated. Radio GC analysis of labeled dephospholylated reaction products revealed FPPS activity in peak area 1 (fractions 15 to 25) and GPPS activity in peak area 2 (fractions 26 to 31) (see Figure 5D).

(B) Immunodetection of GPPS.SSU in the corresponding fractions separated by anion exchange chromatography (A) using the polyclonal antibodies raised against the native GPPS.SSU protein. Crude extract was loaded in the last (right) lane and used as a control (C).