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. 2014 Aug 11;9(8):e104462. doi: 10.1371/journal.pone.0104462

Table 2. Primers for RT-PCR.

Gene Primer Sequences Annealing Temp. °C Cycles Product Size. bp
Transgene T2A Fw: GGAAGTCTGCTAACATGCGGTG 54 36 200
Rv: GGCCATACCATGAAGGCGTTCAT
Rat Oct3/4 Fw: CGAGGCCTTTCCCTCTGTTCCT 62 39 119
Rv: TCTCTTTGTCTACCTCCCTTCCTTGC
Rat Klf4 Fw: CAGACCTGGAAAGTGGTGG 58 39 283
Rv: ACCTGTGTTGCCCGCAGCC
Rat Sox2 Fw: GGCCATTAACGGCACACTGCC 62 39 120
Rv: TTACTCTCCTCTTTTGCACCCCTCC
Rat Eras Fw: CGAGCGGTGTGGGTAAAAGTG 50 36 501
Rv: GGTGTCGGGTCTTCTTGCTTG
Egfp Fw: ATGGTGAGCAAGGGCGAG 58 36 249
Rv: AGTCGTGCTGCTTCATGTGG
β-actin Fw: CATGGCATTGTGATGGACT 53 36 427
Rv: ACGGATGTCAACGTCACACT
Sox17 Fw: GGCACGGAACCCAACCAGC 72 36 210
Rv: CAGTCGTGTCCCTGGTAGGGAAGAC
Ncam Fw: TGCTCAAGTCCCTAGACTGGAACG 72 39 413
Rv: CTTCTCGGGCTCTGTCAGTGGTGTGG
SM22-a Fw: GCTGAAGAATGGCGTGATTCTGAG 62 36 256
Rv: CCTTCAAAGAGGTCAACAGTCTGG
Leptin-R Fw: AACAGCAAAATGATGCAGGG 62 36 322
Rv: GATGCTCAAATGTTTCAGGC