Figure 1.

sil1 Has a Mutation in the AtHDA6 Gene.

The sil1 mutation maps between markers CER456030 and CER455379 at the bottom of chromosome 5. Sequencing of the HDA6 gene in the sil1 mutant reveals a point mutation, 46 bases after the ATG initiation codon, leading to the replacement of Gly₁₆ by Arg. The axe1-5 mutant has a base substitution at position 1635 downstream of the ATG at the third exon-intron junction. Alignment of AtHDA6 with Arabidopsis (At) and human (Hs) RPD3-like HDACs and yeast (Sc) RPD3 reveals a conservation of Gly₁₆ in plant and human RPD3-like HDACs.