Figure 6.

rDNA Expression Is Not Increased in HDA6 Mutant Plants.

Total RNA from control lines DR5 and Ler and mutants axe1-5 and sil1 was subjected to S1 nuclease protection using probes specific for the 5′ end of pre-rRNA transcripts and compared with total RNA amounts, as seen from ethidium bromide staining (A). The signals obtained for rRNA (1) of DR5 and axe1-5 were normalized against signals obtained with probes specific for protein-coding genes ubiquitin (2) or actin (3) (B). All lanes are from the same exposure of the same autoradiogram.