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. 2014 Aug 11;9(8):e104829. doi: 10.1371/journal.pone.0104829

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© 2014 Pang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A: pMG76e-mur; B:pUC19-mur::EryBII. 1: pMG76e-mur was digested by SalI and sphI to 4.2 kb, 3.5 kb, 0.65 kb fragments; 2: Identification of mur gene from pMG76e-mur by PCR with primer 1,2; M: DNA marker DGL4000; 3: Identification of mur gene from pUC19-mur::EryBII by PCR with primer 1,2; 4: pUC19-mur::EryBII was digested by EcorI and sphI to 4.7 kb, 2.7 kb, 2.0 kb fragments.