| Strains |
|
|
|
E. coli DH5α |
F−, φ80d lacZ △M15, △(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rk−, mk+ ), phoA, supE44, λ−, thi-1, gyrA96, relA1 |
TaKaRa |
|
L. bulgaricus ljj-6
|
Wild-type L. bulgaricus isolated from Yogurt samples; low rate of autolysis |
this study |
|
L. bulgaricus 11842 |
Wild-type L. bulgaricus with normalrate of autolysis |
ATCC 11842 |
|
L. bulgaricus k12
|
mur knockout mutant of L. bulgaricusljj-6; mur::EryBII
|
this study |
|
L. bulgaricus O3
|
mur overexpression mutant of L. bulgaricus ljj-6; ljj-6 introductionof a functional mur gene on expression vector pMG76e; |
this study |
| Plasmids |
|
|
| pMD18T |
clone vector; Ampr
|
TaKaRa |
| pMG76e |
pMG36e derived integration vector; Emr
|
College of food science and Nutritional Engineering, China Agricultural University |
| pMD18T-mur |
pMD18T derived integration vector containing the murljj-6 gene with salI, sphI restriction enzyme sites; Ampr
|
this study |
| pMG76e-mur |
pMG76e derived expression vector containing the murljj-6 gene; Emr
|
this study |
| pUC19 |
clone vector; Ampr
|
TaKaRa |
| pUC19-mur |
pUC18 derived integration vector containing the murljj-6 gene with BstEII, HinCII restriction enzyme sites; Ampr
|
this study |
| pUC19-mur::EryBII |
pUC18-mur; Emr; Ampr
|
this study |
