Figure 3.

Analysis of AtORC2 Expression.

(A) Assay for AtORC2 and actin11 transcripts by RT-PCR: lane 1, DNA size markers; lane 2, large PCR product from plasmid p1300-ORC2 that contains a genomic fragment spanning the complete AtORC2 gene; lane 3, control (no CDNA); lanes 4 to 6, PCR products from cDNA of seedlings (lane 4), mature leaves (lane 5), and inflorescences (lane 6).

(B) to (E) In situ hybridization experiments. CC, central cell; E, endosperm; EC, egg cell; S, synergid.

(B) Female gametophyte, antisense probe.

(C) Female gametophyte, sense probe.

(D) Preglobular embryo, antisense probe.

(E) Preglobular embryo, sense probe.

(F) and (G) GUS activity assays on tissue from orc2/+ plants.

(F) GUS activity in the trichome.

(G) GUS activity in pollen tubes from an orc2/+ plant on a wild-type stigma.