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. 2004 Apr;16(4):1047–1059. doi: 10.1105/tpc.019406

Figure 8.

Figure 8.

Cytoplasmic Localization of MtRBP1 in the Presence of the enod40 RNA.

(A) to (C) Cobombardments with MS2coat-GFP, MtRBP1-DsRed2, and the following MS2-RNA constructs: MS2-enod40 RNA (600 nucleotides) (A), MS2-Mtapk1 RNA (1700 nucleotides) (B), and no hybrid RNA (C). Cells were observed for GFP and DsRed2 fluorescence, which label hybrid MS2-RNA molecules and the MtRBP1 protein, respectively. Note that red fluorescence can be detected in cytoplasmic granules only in the presence of enod40 (top right panel). N, nucleus; C, cytoplasm. Scale bars are indicated.

(D) Cobombardment of MtRBP1-DsRed2, MS2coat-GFP, and MS2-enod40 RNA. Detail of a merged image of the cytoplasm with the corresponding spectral fluorescence profile (obtained along the indicated white line in the image), showing colocalization between the enod40 RNA (green) and the MtRBP1 protein (red). The arrow indicates the presence of enod40 RNA (green) not associated to MtRBP1 (red) in the cytoplasm. Note that all granules are green or yellow but never red (MtRBP1 alone was not detected in the cytoplasm).