Table 2.

Long-term stability of HSPC/CH/mPEG₂₀₀₀-DSPE (3/1/1, w/w/w) liposomes loaded with doxorubicin (drug/lipid ratio 1:10, w/w) via 200 mM NH₄EDTA and (NH₄)₂SO₄ gradient stored at 4°C±2°C

Day	NH4EDTA-L			(NH4)2SO4-L
	Appearance	Particle size ± SD (nm; n =3)	Entrapment efficiency ± SD (%; n=3)	Appearance	Particle size ± SD (nm; n=3)	Entrapment efficiency ± SD (%; n=3)
0	+	79.4±1.87	95.54±0.59	+	76.9±2.15	95.07±0.78
15	+	80.9±2.83	97.29±1.16	+	82.5±8.00	95.92±0.74
30	+	87.1±3.25	96.02±1.35	+	87.4±5.48	95.52±0.78
60	+	93.1±4.85	95.60±1.04	↓	–	–
90	+	106.2±3.55	95.32±1.05	↓	–	–

Notes: + represents cherry red liposomes, clear, without precipitation, phase separation, and bacterium; ↓ represents cherry red liposomes, precipitation, or bacterium; and – represents no data.

Abbreviations: CH, cholesterol; EDTA, ethylenediaminetetraacetic acid; HSPC, hydrogenated soy phosphatidylcholine; L, doxorubicin-loaded liposomes; mPEG₂₀₀₀-DSPE, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy[polyethylene glycol]-2000); SD, standard deviation; w/w, weight/weight.