Table 2. Inhibitor Ki values with AA from Dixon replots.a .
Compound | Ki | Ki′ |
MLS000545091 | 0.9 (0.4) | 9.9 (0.7) |
MLS000536924 | 2.5 (0.5) | n/ab |
All assays were done in triplicate.
The Ki was determined from the slope (Km/Vmax) replot at varying inhibitor concentration. The Ki′ was determined similarly from the y-intercept (1/Vmax) replot.
The y-intercept did not vary with increasing inhibitor, indicating a competitive inhibitor.
The inhibition constants are in units of micromolar, with error in parentheses.