Figure 8. CO blocks Mb-induced myosin oxidation.

A: Myosin oxidation is followed by fluorescence intensity of bityrosines formed. Reaction mixtures contained My (0.2 µM), oxyMb (0.5 µM) and H₂O₂ (200 µM). (25°C, Ex/Em = 327/410 nm, n = 5.); B: Myosin (2.5 µM) oxidation by Mb is followed by formation of covalent high molecular weight aggregates as demonstrated by SDS-PAGE. 1: My alone and also in presence of H₂O₂ (control). 2: Mb alone and also in presence of H₂O₂ (control). 3–5: oxyMb (30 µM)+H₂O₂ (200 µM); pH 7.3; 40 min incubation in air (3); under N₂ (4); under CO (5).