Figure 8. Bik mediates synergism by carfilzomib/vorinostat in proteasome inhibitor-resistant cells. (A) R-Cal33 cells were seeded in 24-well plates, grown overnight, then transfected for 6 h with nonspecific siRNA or Bik siRNA. The transfected cells were then treated for 36 h with the indicated concentrations of CFZ alone, VOR alone, or the CFZ/VOR combination. Following treatment, the floating and attached cells were collected and combined, then subjected to flow cytometric analysis of Annexin V/PI staining. A representative experiment is shown, with numbers indicating the percentage of Annexin V-positive cells. The experiment was performed 3 times with similar results. (B) Data from the three independent experiments performed in (A) were combined and graphed. Columns represent the mean of values obtained in the 3 experiments, and error bars represent standard deviations.