Figure 3. The regulation of cell motility by Arp2 in gastric cancer cells. (A) Three siRNA pairs targeting Arp2 were synthesized, and their inhibitory effects were examined by western blotting. (B) Wound-healing assays showed that Arp2 knockdown decreased the wound closure rate. (C) Arp2 suppression inhibited the migration and invasion of MKN28-M cells. (1) Representative images of the migration and invasion assays; (2) The relative cell motility activities of cells. (D) Western blot analysis for Arp2 following transfection with the Arp2 expression plasmid or empty vector into MKN28-NM cells. (E) Wound-healing assay showed that Arp2 upregulation increased the wound closure rate. (F) Ectopic expression of Apr2 promoted the migration and invasion of MKN28-NM cells. (1) Representative images of the migration and invasion assays; (2) The relative cell motility activities of cells.