Figure 2. Identification of bioactivity for the IL-2 and TRAIL expressed by rNDV. (A) T (CTLL2) cells proliferation was stimulated by IL-2 expressed by rNDV-IL2 or rNDV-IL-2-TRAIL-infected DF1 cells. (B) The purified IL-2 enhanced cytotoxicity of immune cells for H22 and B16-F10 cells. Student’s paired two-tailed t test revealed a significant effect. **P < 0.01, vs control. (C–F) The purified TRAIL from rNDV-TRAIL or rNDV-IL-2-TRAIL-infected DF1 cells inhibited tumor cells growth. (G–J) TRAIL inhibited tumor cells growth in vitro in a concentration-dependent manner. ANOVA revealed a significant effect. *P < 0.05, **P < 0.01, vs control. All the values are the mean and SEM of triplicate samples.