Figure 6. Effects of combination of I3C and Adhz63 on induction of apoptosis in A549 cells. Cells were pre-treated with I3C for seven days and then infected with Adhz63 at a MOI of 1 for three days. (A) Flow cytometry analysis was performed using a dual staining approach with 7-AAD and Annexin V for detection of co-treatment induced apoptosis. (B) Caspase 9 activities were determined by caspase-9 Colorimetric Assay. Co-treatment of I3C and Adhz63 vs. I3C alone control correspondence (*P < 0.05, **P < 0.001); vs. Adhz63 alone control (^†P < 0.05). (C) The protein levels of cleaved caspase 9, caspase 3, and PARP in A549 cells were determined by western blot analysis.