Figure 1. CCNB1 is overexpressed in colorectal cancer. (A) qRT-PCR analysis of CCNB1 mRNA expression in 30 paired human colorectal cancer tissues and the matched adjacent normal tissues. GAPDH was used as internal control and for normalization of the data. The data shown were average fold changes of CCNB1 expression (2^−ΔCT) in colorectal cancer tissues relative to the matched adjacent normal tissues. (B) Western blot validation of CCNB1 protein in the 30 paired human colorectal cancer tissues and the matched adjacent normal tissues. CCNB1 protein expression was normalized to GAPDH and expressed relative to the matched adjacent normal tissues. (C) Expression of CCNB1 protein by western blot in five colon cancer cell lines. Data were presented as mean ± SD.