Figure 1.

Ras and PKC signaling in ST cells. (A) Cell lysates were extracted from ST8814 and ST/Nf1 cells and subjected to Ras Pull-Down assay. The even loadings of total proteins were normalized by Ras expression. (B) Cell lysates were prepared and then immunoblotted with the anti-phosphorylated Akt or ERK1/2 antibody. The even loadings of total proteins were normalized by Akt or ERK1/2 expression. (C) After the treatments of PMA, HMG, or both, cell lysates were subjected to immunoprecipitation with an anti-PKC antibody. PKC activity in the immunoprecipitates was then analyzed using a PKC enzymatic kit. The error bars represent SD from 5 independent experiments (n = 5, P values < 0.05).