Figure 3.

Persistent mitotic arrest and mitotic catastrophe occurred in HMG-treated ST8814 cells. (A) ST8814 or SNF02.2 cells were treated with HMG for 32 h, cell cycle analysis was performed using a flow cytometer. The percentages of the cells accumulated in the G₂ and M phases were plotted (left panel). The error bars represent SD from 5 independent experiments (n = 5, * P values < 0.05). The DNA profiles of the untreated or HMG–treated ST8814 and ST/Nf1 cells were presented in the right panels. (B) Thirty-two hours after HMG treatment, the cells were stained with Giemsa dye and photos of the stained nuclei were taken. (C) With or without HMG treatment, cell lysates were prepared and subjected to immunoblotting with an anti-cyclin B1 antibody. The even loadings of total proteins were normalized by actin expression.