Figure 2. (A) Hepatic expression of Keap1 protein during the first wave of hepatocyte proliferation after partial hepatectomy (PH) in Keap1+/+ and Keap1+/− mice. Total liver lysates were prepared from the livers collected at the indicated time points after PH. Western blotting was performed with an antibody against Keap1 protein. Glyceraldehyde 3-phosphate dehydrogenase (GADPH) was used as a loading control. A representative blot of 3 independent experiments is shown. NL, normal liver. (B) Hepatic mRNA expression of Nrf2, NQO1, GPX2, GSTmu3, and Klf9 during the first wave of hepatocyte proliferation after PH in Keap1+/+ and Keap1+/− mice. Total RNA was prepared from liver tissues collected prior to or after PH at the indicated time points. Hepatic mRNA levels for the genes indicated were measured by qRT-PCR and are expressed as the mean fold changes compared with wild-type normal controls ± SD (n = 3 mice per time point per genotype). Asterisks represent P < 0.05 in comparison between Keap1+/+ and Keap1+/− mice.