Figure 4. Protein expression of a subset of mitogenic signaling molecules in regenerating livers of Keap1+/+ and Keap1+/− mice. Liver lysates were prepared as described in Figure 3. Western blotting was performed with antibodies against the proteins indicated. GADPH was used as a loading control. Blue and red boxes indicate the similar phosphorylation patterns of p-Akt1 (T308) and p-p70S6K (T389) in Keap1+/+ and Keap1+/− regenerating livers, respectively.