Figure 4. Knockdown of Tpm3 impairs cortical granule-free domain (CGFD) and actin cap formation in mouse oocytes. (A) In control small interfering RNA (siRNA)-injected oocytes, cortical granules are absent from the cortex close to where chromosomes are located during metaphase II (MII). Conversely, in Tpm3-targeting siRNA-injected oocytes, cortical granules are distributed throughout the entire cortex. Green, cortical granules; blue, DNA. (B) Status of CGFD formation in control siRNA-injected and Tpm3-targeting siRNA-injected oocytes. (C) Failure of cortical actin cap formation in Tpm3-targeting siRNA-injected oocytes.