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. 2014 Jun 19;13(15):2391–2399. doi: 10.4161/cc.29337

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Copyright © 2014 Landes Bioscience

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Figure 3. — EWS colocalizes with Aurora B at the midzone in A673 and HeLa cells. Single Z-section images of (A) Top, merged images with DNA stained using DAPI (blue), Aurora B (red) visualized using anti-Aurora B antibody, and EWS (green) via anti-EWS antibody; middle, Aurora B visualized with anti-Aurora B antibody; bottom, EWS visualized with anti-EWS antibody in A673 cells; (B) top, merged images with DNA stained with DAPI (blue), Aurora B (red) visualized with anti-Aurora B antibody and EWS (green) with anti-EWS antibody; middle, Aurora B visualized with anti-Aurora B antibody; bottom, EWS visualized with anti-EWS antibody in HeLa cells. Higher magnification images of midzone are shown in the boxed area in anaphase images obtained from both A673 and HeLa cells (A and B).