Figure 11. c-Jun promotes TGFβ-induced invasion of prostate cancer cells. (A) Knock-down of c-Jun in PC-3U cells significantly prevented TGFβ-induced invasion. Data are presented as mean values (± S.D.) for invasive cells in 3 independent experiments (***, P < 0.001, when compared with control siRNA). (B) Invasion assay was performed in PC-3U cells transiently transfected with control (CTRL) or c-Jun siRNA, and treated as indicated. Cells were then visualized by staining with crystal violet cell stain solution. (C and D) Invasion assay was performed in PC-3U cells transiently transfected with CTRL, c-Jun siRNA alone or together with HA-tagged Snail1, and treated as indicated. (E) Cell lysates derived from a part of the cells in (C) was subjected to immunoblotting for total c-Jun and HA (Snail). Actin served as internal control for equal loading of proteins. (F) The number of proliferating PC-3U cells transiently transfected with control (CTRL) or c-Jun siRNA, and treated as indicated, was subjected to immunofluorescence stainings of phospo-Histone3 (p-H3). Data are presented as mean values (± S.D.) in 3 independent experiments. (**, P < 0.01, ***, P < 0.001, when compared with control siRNA).