Extended Data Table 2.

Binding affinities for different P2Y12R constructs. Affinity values of the agonist [³H]2MeSADP determined in saturation binding to WT and mutant P2Y12Rs expressed transiently in COS7 cells (a) and inhibition by antagonist AZD1283 (b).

a
Constructs	[3H]2MeSADP(Kd, nM)
WT	4.9±1.3
S83A	5.6±1.4
C97A	N.S.
R256A	16.1±6.2
C175A	N.S.
K280A	N.S.

b
Constructs	AZD1283 (Ki, nM)
WT	41.8±16.3
S83A	36.5±6.8
R256A	140±39

Footnote: Results are expressed as mean ± SEM from 3-6 independent experiments performed in duplicate by methods described in Zhang et al5. N.S., not saturable or negligible specific binding within the radioligand concentrations used (0.4-46 nM).