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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Mol Psychiatry. 2014 Feb 11;19(12):1275–1283. doi: 10.1038/mp.2013.190

Figure 4.

Figure 4

Cort treatment induces a pro-oligodendrogenic transcriptional program in NSCs. (a) Fold change in mRNA expression of genes regulating neurogenic and oligodendrogenic fate in cort-treated NSCs, relative to vehicle-treated controls, including genes that promote (+) and inhibit (−) oligodendrogenesis. (b) Fold change in mRNA expression of oligodendrogenic regulatory genes in NSCs transfected with dnGR or GFP (control) vectors, or no vector, and treated with cort, relative to vehicle-treated controls; n≥3. (c) Representative image of Western blot for Olig1 protein in NSCs treated with cort or vehicle. (d) Densitometric analysis of Western blot for total protein fraction and (e) the isolated nuclear fraction of treated NSCs; n=3. (f) IHC analysis of the percent of BrdU positive cells expressing nuclear-localized Olig1 in the hilus of adult rats after 1 week of daily cort injections; n=6 vehicle injected, n=6 cort injected (g) or after 1 week of daily immobilization stress; n=5 no stress control, n=6 stress. *p < 0.05, **p<0.005, ***p < 0.0001 (mean ± SEM).