Figure 1.

MAGL expression in different NPC cells and tissues. A. The mRNA level of MAGL (normalized to GAPDH) in the 6 NPC cell lines were measured by quantitative real–time PCR, showing that the expression of MAGL was significantly higher in high metastatic potential cells, S18, than in its parental low metastasis cell line, CNE-2. Moreover, high levels of mRNA were also observed in another high-metastatic clone, 5-8F, in contrast to its parental low metastatic NPC cell line SUNE-1. NP 69, an immortalized nasopharyngeal epithelial cell line exhibited a relatively low expression of MAGL. Column, mean; error bar, ± SD (from triplicates). B. The MAGL protein levels in these cell lines were confirmed by western blotting. C. The mRNA level of MAGL (normalized to GAPDH) in 10 NPC patients was significant higher than that in 10 non-cancer patients with rhinitis (***, p < 0.001).