Figure 4.

In vivo metastasis rates of MAGL-overexpressing cells and MAGL knockdown cells. For overexpressing cells, CNE-2-MAGL-OE and vector cells were subcutaneously injected into the left hind footpad of nude mice as well as SUNE-1-MAGL-OE and its vector cells, which led to metastases to the left popliteal LN in some animals. For the knockdown cells, S18 and 5-8F cells stably expressing MAGL shRNAs (SH 1 and SH 2) or scrambled shRNA (NC) were used. A. The proportion of popliteal LN metastasis after inoculation of CNE-2 cells. Overexpression of MAGL in CNE-2 cells significantly increased the metastasis rate from 16.67% (5 of 30) to 60% (18 of 30). B. The proportion of popliteal LN metastasis after inoculation of SUNE-1 cells. Overexpression of MAGL in SUNE-1 cells significantly increased the metastasis rate from 20% (6 of 30) to 63.33% (19 of 30). C. Knockdown of MAGL in S18 cells significantly reduced the metastasis rate from 76.67% (23 of 30) to 40% (12 of 30). D. The proportion of popliteal LN metastases after inoculation of 5-8F cells. Knockdown of MAGL in 5-8F cells significantly reduced the metastasis rate from 73.33% (22 of 30) to 40% (12 of 30). χ² test was used.