Figure 4.

Overexpression of LAP3 promotes Huh7 cell proliferation and migration abilities in vitro. A. Western blot analysis of LAP3, PCNA, E-cadherin, GAPDH (loading control) in control and myc-LAP3 Huh7 cells. The bar chart showed LAP3 expression ratio to GAPDH. The data are mean ± SEM (n = 3, *P<0.01, compared with control). B. Effect of LAP3 up-regulation on proliferation of Huh7 cells, analyzed by the cell viability assay. Data are representative of three independent experiments. *, P<0.05. C. Up-regulation of LAP3 could promote G1/S transition in Huh7 cells. The cell cycle distribution of Huh7 cells detected by flow cytometric analysis. D. Wound healing assays with negative control and myc-LAP3 Huh7 cells. Migration of the cells to the wound was visualized at 0, 48 h with an inverted Leica phase-contrast microscope. The bar chart showed the relative migration distance of cells. The data are mean ± SEM (n = 3, *P<0.05, compared with 0 h). E. Up-regulation of LAP3 promoted cell migration by transwell assays. For transwell assays, control and myc-LAP3 Huh7 cells were seeded into the upper chamber of the transwell and the cells that migrated through the pores to the lower surface of the filter were counted in 10 fields under 20× objective lens. The bar chart showed the percentage of migrant cells. The data are mean ± SEM (n = 3, *P<0.01, compared with control).