Figure 5.

Downregulation of LAP3 increased the sensitivity of HCC cells to cisplatin. A. Western blot analysis indicated the dose-dependent effect of cisplatin on the LAP3 expressions in HepG2 cells, which were treated by cisplatin at different concentrations for 48 h. GAPDH was used as a control for protein load and integrity. B. The bar chart showed the expression ratio to GAPDH. The data are mean ± SEM (n = 3, *P<0.01, compared with 0h). C. Western blot analysis indicated the effects of cisplatin on the LAP3 expression in HepG2 cells, which were transfected with control or shRNA-LAP3 for 24 h, respectively, and then subjected to treatment with or without cisplatin for 48 h, respectively. GAPDH was used as a control for protein load and integrity. D. A bar chart showed that the comparison in the cell growth rate in HepG2 cells, which were transfected with control or shRNA-LAP3 for 24 h, respectively, and then subjected to treatment with or without cisplatin for 48 h, respectively, as measured by cell viability assays. Data were presented as mean ± SEM. *P<0.05, versus controls (non-treated with cisplatin), respectively.