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. 2014 Jun 15;7(7):3938–3947.

Figure 3.

Figure 3

The change of GLUT-1 mRNA and protein expression. A: At 48 and 72 h, apigenin downregulated the expression of GLUT-1mRNA in a concentration-dependent manner (P < 0.01). At the same apigenin concentration, the GLUT-1mRNA level was reduced significantly upon prolongation of culture duration (P < 0.01). B: At 72 h, there was no significant difference in GLUT-1 mRNA level between cultures with 4 and 5 μg/ml cisplatin. The GLUT-1 mRNA level at 72 h of culture was higher than at 48 h of culture in the presence of 4 and 5 μg/ml cisplatin. C: Apigenin significantly reduced the GLUT-1 mRNA level in cisplatin-treated Hep-2 cells in a concentration- and time-dependent manner (P < 0.01). C: Western blotting revealed that apigenin or cisplatin alone did not significantly reduce the GLUT-1 protein levels (P > 0.05). D: Significant decreases in GLUT-1 protein levels were noted after 48 h of exposure to 40 and 160 μM apigenin of cisplatin-treated Hep-2 cells (P <0.01 ). E: The results of Western blotting.