Table 1.

Primers and conditions of RT-PCR analysis

Genes	Primers from 5’ to 3’	Annealing temperature	Cycle no.	Product size (bp)
MAGE-A1	f: GGAGCACCAAGGAGAAGA	57°C	35	291
	r: TGATGGTAGTGGGAAAGG
MAGE-A3	f: AGTCCGAGTTCCAAGCAG	53°C	35	239
	r: GCAGGTGGCAAAGATGTA
MAGE-A12	f: GGAAGATGGCTGAGTTGG	53°C	35	214
	r: AGGCAGGTGACAAGGATG
cTAGE-1	f: GGAGACCTACCGAAAGCG	53°C	35	303
	r: TCAGATGAAGGCCAACCC
cTAGE-2	f: GGAGACCTACCGAAAGCG	53°C	35	303
	r: TCAGATGAAGGCCAACCC
NY-ESO-1	f: GAGCCGCCTGCTTGAGTT	53°C	35	245
	r: AGCACTGCGTGATCCACATC
β-actin	f: GTGGGGCGCCCCAGGCACCA	53°C	35	498
	r: CTCCTTAATGTCACGCACGATTTC