Figure 2.

Gliomatous areas are irregularly intermingled with sarcomatous components composed of spindle-shaped cells (HE A, × 200).Gliomatous component demonstrates tumor cells with abundant cytoplasm and eccentrically placed (HE B, × 400, arrow) Ki-67 labelling index low in granular cell astrocytoma tumor cells (C, × 400). Sarcomatous areas were composed predominantly of spindle-shaped cells arranged in intersecting fascicles in herringbone and storiform patterns (HE D, × 400, arrow). Sarcomatous areas were strongly positive for reticular fiber staining (E, × 100). Ki-67 labelling index high in sarcomatous areas tumor cells (F, × 200). Double staining for vimentin and GFAP showed that granular cell astrocytoma tumor cells were all GFAP-positive (pink), and sarcomatous areas cells were all Vimentin-positive (brown). (G, × 400); Granular cell astrocytoma tumor cells were all PAS-positive (H, × 400) and CD68-positive (I × 200).