Table 1. Characteristics that differentiate strains ACB1Tand ACB8 and strain ACB7T from O. sinus AIP 354.02T (Carlier et al., 2004).
Characteristic | 1 | 2 | 3 | 4 |
Cell shape | Short ovoid rods in chains or aggregates, often swollen | Short ovoid rods in chains or aggregates, often swollen | Short ovoid to long curved rods occurring singly, in pairs or chains, often swollen | Ovoid rods occurring singly, in pairs or in chains |
Cell size (μm) | 1.2±0.4×0.45±0.09 | 1.2±0.4×0.45±0.09 | 1.6±0.6×0.5±0.08 | 1.7–2.2×0.8–1.0 |
Motility/flagella | +/Sublateral | +/Sublateral | +/Sublateral | +/Laterally inserted |
Colony shape | Round, convex, beige | Round, convex, beige | Round, transparent, petite | Circular, convex, non-pigmented |
Colony size after 48 h (mm) | 1.5 | 1.5 | 0.5 | 1.5 |
Gram-stain | Variable | Variable | Variable | Negative |
Temperature range (°C) | 30–42 | 30–42 | 30–42 | 37 |
Assimilation of: | ||||
d-Glucose | + | + | − | + |
Lactose | + | + | − | − |
Sucrose | − | + | + | + |
Maltose | + | + | − | − |
Maltodextrin | + | + | − | nd |
Raffinose | + | + | − | + |
Indole formation | − | nd | − | + |
Urease | − | nd | − | nd |
Oxidase | − | − | − | nd |
Aesculin hydrolysis | − | nd | + | − |
Gas formation from TGY medium | + | + | − | + |
Black pigment on TY medium | + | + | + | nd |
Resistance to: | ||||
Kanamycin (1 mg) | − | nd | − | + |
Erythromycin (60 µg) | − | nd | − | + |
Metabolic end products* | A, L | A | A | A, L |
DNA G+C content (mol%) | 42.1 | 42 | 43.3 | 42.4 |
Peptidoglycan type | A1γ | nd | A1γ | nd |
Major fatty acid methyl esters | C14 : 0 | nd | C12 : 0 | C14 : 0 |
C16 : 0 | C14 : 0 | anteiso-C15 : 0 | ||
C16 : 1ω7c DMA | C16 : 0 | C15 : 0 | ||
C18 : 1ω7c DMA | C16 : 1ω7c | C16 : 1ω9c | ||
C16 : 1ω7c DMA | C16 : 0 | |||
Isolation source | Human subgingival plaque | Human subgingival plaque | Human subgingival plaque | Human maxillary sinus |
A, acetate; L, lactate.