Fig. 4.

WFA increases the expression of p53 and p21. (A) Chondrocytes were untreated or treated with the indicated concentrations of WFA for 24 hr. (B) Chondrocytes were untreated or treated with 5 µM WFA for the indicated time periods. (C) Cells were untreated or treated with 5 µM WFA in the absence or presence of 5 mM NAC for the specified time periods. (D) Chondrocytes were untreated or treated with 5 µM WFA in the absence or presence of the increased concentrations of NAC for 24 hr. (E) Cell were untreated or treated with 5 µM WFA in the absence or presence of the indicated concentrations of L-NMMA for 24 hr. (A-E) Expressions of p53 and p21 were analyzed by Western blot analysis. Actin was used as loading control. The data are representative result of three similar experiments. Values are means ± SEM of three experiments (n = 3); statistically significant: ^*P < 0.01 (versus the corresponding untreated group).