Fig. 3.

Localizations of centromeric proteins in CENP-U and CENP-R-deficient ES cells. a Immunofluorescence analysis of wild-type, CENP-U-deficient ES cells (+OHT 48 h) using antibodies against CENP-R, CENP-U, CENP-O, and CENP-H. Bar, 10 μm. b Western blot analysis of CENP-U-deficient cells with antibodies against CENP-U, CENP-R, CENP-O, and CENP-H. Anti-tubulin antibody was used as a loading control. The amounts of CENP-U, CENP-R, and CENP-O were decreased in the CENP-U-deficient cells but not those of CENP-H. c A summary of the association of CENP-O-class proteins. CENP-O, CENP-P, CENP-Q, and CENP-U form a stable complex. This stable complex functions downstream of CENP-H-associated proteins and upstream of CENP-R