Figure 2. Phylogenetic relationships based on the VP1, P1, P2, and P3 genome regions of enterovirus B (EV-B).

Two Tibetan EV-B81 strains (indicated by solid circles) and 55 other EV-B prototype strains were analyzed by nucleotide sequence alignment using the Neighbor-Joining algorithms implemented in the MEGA 5.0 program. Numbers at the nodes indicate bootstrap support for that node (percent of 1000 bootstrap replicates). The open triangle indicates the India EV-B81 which has the entire VP1 sequence in the GenBank database, and the solid diamond indicates EV-B81 prototype strain. The scale bars represent the genetic distance. All panels have the same scale. (a) VP1 coding sequences; (b) P1 coding sequences; (c) P2 coding sequences; and (d) P3 coding sequences.